[Biochemical Engineering Journal] Transfection of Jurkat T cells by droplet electroporation
A droplet electroporation system has been successfully demonstrated for transfection of Jurkat T cell with much higher transfection efficiency (66%) than that of conventional system (11.4%) with the advantages of superior cell viability, comparable throughput, and user-friendly interface. Because the productivity of the proposed system is much greater than previous microfluidic systems, flow cytometry is used for the analysis of transfection efficiency. The high transfection efficiency and cell viability of the droplet electroporation system is mainly attributed to small size which requires lower voltage and provides concentrated environment. The successful transfection of Jurkat cell using the droplet electroporation system broadens the applicability of the proposed technology to medical field. The implication of the present work and the future development direction for a fully automated cell engineering platform are discussed.
Do Jin Im a, Su-Nam Jeong b a Department of Chemical Engineering, Pukyong National University, Sinseon-ro, Nam-Gu, Busan 608-739, South Korea b Department of Chemical Engineering, Pohang University of Science and Technology, San 31, Hyoja-Dong, Nam-Gu, Pohang, Gyeongbuk 790-784, South Korea Received 11 September 2016, Revised 15 March 2017, Accepted 16 March 2017, Available online 18 March 2017