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[Journal of Analytical Methods in Chemistry] Acupuncture Injection Combined with Electrokinetic Inje


Abstract:

We recently reported acupuncture sample injection that leads to reproducible injection of nL-scale sample segments into a polydimethylsiloxane (PDMS) microchannel for microchip capillary electrophoresis. The advantages of the acupuncture injection in microchip capillary electrophoresis include capability of minimizing sample loss and voltage control hardware and capability of introducing sample plugs into any desired position of a microchannel. However, the challenge in the previous study was to achieve reproducible, pL-scale sample injections into PDMS microchannels. In the present study, we introduce an acupuncture injection technique combined with electrokinetic injection (AICEI) technique to inject pL-scale sample segments for microchip capillary electrophoresis. We carried out the capillary zone electrophoresis (CZE) separation of FITC and fluorescein, and the mixture of 10 μM FITC and 10 μM fluorescein was separated completely by using the AICEI method.

Ji Won Ha Department of Chemistry, University of Ulsan, 93 Daehak-Ro, Nam-Gu, Ulsan 44610, Republic of Korea

Correspondence should be addressed to Ji Won Ha

Received 15 January 2017; Revised 9 February 2017; Accepted 12 February 2017; Published 23 February 2017

Academic Editor: Chih-Ching Huang

Copyright © 2017 Ji Won Ha. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Journal of Analytical Methods in Chemistry Volume 2017 (2017), Article ID 7495348, 6 pages https://doi.org/10.1155/2017/7495348

Link: https://www.hindawi.com/journals/jamc/2017/7495348/

#03232017 #capillary #electrophoresis #microchannels

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