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[Scientific Report] Microscale Symmetrical Electroporator Array as a Versatile Molecular Delivery Sy


Abstract:

Successful developments of new therapeutic strategies often rely on the ability to deliver exogenous molecules into cytosol. We have developed a versatile on-chip vortex-assisted electroporation system, engineered to conduct sequential intracellular delivery of multiple molecules into various cell types at low voltage in a dosage-controlled manner. Micro-patterned planar electrodes permit substantial reduction in operational voltages and seamless integration with an existing microfluidic technology. Equipped with real-time process visualization functionality, the system enables on-chip optimization of electroporation parameters for cells with varying properties. Moreover, the system’s dosage control and multi-molecular delivery capabilities facilitate intracellular delivery of various molecules as a single agent or in combination and its utility in biological research has been demonstrated by conducting RNA interference assays. We envision the system to be a powerful tool, aiding a wide range of applications, requiring single-cell level co-administrations of multiple molecules with controlled dosages.

Mengxing Ouyang, Winfield Hill, Jung Hyun Lee & Soojung Claire Hur

Scientific Reports 7, Article number: 44757 (2017) doi:10.1038/srep44757

Link: http://www.nature.com/articles/srep44757

#03212017 #therapeutic #electroporation #electrode #molecule

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