[Chemical Communications] Site-Specific Antibody Modification and Immobilization on a Microfluidic C
We have designed and synthesized EpCAM antibodies with Fc-domain site-specific linkers in allowing preferential alignment when coated on microfluidic device for capturing circulating tumor cells (CTCs) from colorectal cancer patients. The aligned coating has shown to increase capture efficiency of CTCs and microemboli by 1.6 and 3.0 -fold, respectively (both P < 0.05).
Chian-Hui Lai, Syer Choon Lim, Liang-Chun Wu, Chien-Fang Wang, Wen-Sy Tsai, Han-Chung Wub and Ying-Chih Chang
Chem. Commun., 2017, Accepted Manuscript
DOI: 10.1039/C7CC00247E Received 12 Jan 2017, Accepted 15 Mar 2017 First published online 17 Mar 2017