[Thesis] Microluidic Sorting of Blood Cells by Negative Selection
In this study, a microfluidic cell sorter device based on passive inertial microfluidic channel was developed to overcome the limitations of conventional cell sorter devices. An inertial microfluidic cell sorter was designed and optimized to successfully sort particles with high throughput. Sorting a mixture of 15 µm and 7 µm diameter particles shows a >99% sorting efficiency. This is a significantly higher efficiency than the conventional macroscale devices. After successfully sorting mixed particles, 5 µm diameter particles and 5× diluted blood sample mixture solution wereused to demonstrate separation of a real biological sample. Specifically, microparticles were functionalized to target CD45 surface marker present on white blood cells to select these non-target cells and “enlarge” their diameter for removal. The protocols for immuno-coating of microparticles and their binding to CD45+ cells were optimized in order to increase binding efficency to 68.9%. Separation of blood cells was demonstrated with 99.6% efficiency. Ultimately, we envision the use of this novel approach in isolation of rare circulating cells from blood in point-of-care and biomedical applications.
Year and Degree
2016, MS, University of Cincinnati, Engineering and Applied Science: Electrical Engineering.
Ian Papautsky, Ph.D. (Committee Chair) Chong Ahn, Ph.D. (Committee Member) Leyla Esfandiari, Ph.D. (Committee Member)