[Integration, the VLSI Journal] A computational design of a versatile microchamber for in vitro nano
The emergence of nanosecond pulsed electric fields (nsPEFs) for intracellular manipulation experiments requires the use of specific miniaturized applicators. We propose the design of a versatile nsPEFs applicator, based on microwave propagating systems, suitable for in vitro exposure to undistorted 1–3 ns pulses in single and multi-cell experiments. Further features of the proposed devices are: high efficiency, microfluidic integration, real time monitoring of the biological sample and of the pulse propagation. Generally, these features can be considered as specific requisites for nanosecond applicators, to ensure experimental repeatability and reproducibility, when propagation related phenomena can’t be considered negligible.
Maura Casciolaa, b, 1, Micaela Libertia, Agnese Denzia, b, Alessandra Paffia, Caterina Merlac, Francesca Apollonioa, , a Department of Information Engineering, Electronics and Telecommunications (D.I.E.T.), Sapienza University of Rome, via Eudossiana 18, 00184, Rome, Italy b Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Viale Regina Elena 291, 00161, Rome, Italy c Division of Health Protection Technologies, ENEA, via Anguillarese 301, 00123, Rome, Italy Available online 12 March 2017 http://dx.doi.org/10.1016/j.vlsi.2017.03.005