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[Conference Article] Preliminary assessment for DNA extraction on microfluidic channel


Abstract:

The aim of this research is to extract, purify and yield DNA in mushroom from solid state mushroom sample by using fabricated continuous high-capacity sample delivery microfluidic through integrated solid state extraction based amino-coated silica bead. This device is made to specifically extract DNA in mushroom sample in continuous inflow process with energy and cost consumption. In this project, we present two methods of DNA extraction and purification which are by using centrifuge (complex and conventional method) and by using microfluidic biosensor (new and fast method). DNA extracted can be determined by using ultraviolet-visible spectroscopy (UV-VIS). The peak obtained at wavelength 260nm after measuring the absorbance of sample proves that DNA is successfully extracted from the mushroom.

Subash C. B. Gopinath1,2,a), Uda Hashim1, and M. N. A. Uda2

a)Corresponding author: subash@unimap.edu.my

  • 1Institute of Nano Electronic Engineering, University Malaysia Perlis (UniMAP), 01000 Kangar, Perlis, Malaysia

  • 2School of Bioprocess Engineering, Universiti Malaysia Perlis, Kompleks Pusat Pengajian, Jejawi 3,02600 Arau, Perlis, Malaysia

AIP Conference Proceedings 1808, 020018 (2017); doi: http://dx.doi.org/10.1063/1.4975251

Link: http://aip.scitation.org/doi/abs/10.1063/1.4975251

#03172017 #biosensor #silicabead #Biologicalapplication

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