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[Microfluidics and Nanofluidics]From single-molecule detection to next-generation sequencing: microf


Abstract:

Droplet-based microfluidic technologies have proved themselves to be of significant utility in the performance of high-throughput chemical and biological experiments. By encapsulating and isolating reagents within femtoliter–nanoliter droplet, millions of (bio) chemical reactions can be processed in a parallel fashion and on ultra-short timescales. Recent applications of such technologies to genetic analysis have suggested significant utility in low-cost, efficient and rapid workflows for DNA amplification, rare mutation detection, antibody screening and next-generation sequencing. To this end, we describe and highlight some of the most interesting recent developments and applications of droplet-based microfluidics in the broad area of nucleic acid analysis. In addition, we also present a cursory description of some of the most essential functional components, which allow the creation of integrated and complex workflows based on flowing streams of droplets.

Yun DingJaebum ChooAndrew J. deMello

Ding, Y., Choo, J. & deMello, A.J. Microfluid Nanofluid (2017) 21: 58. doi:10.1007/s10404-017-1889-4

Link: https://link.springer.com/article/10.1007/s10404-017-1889-4

#031317 #dropbasedmicrofluidics #DigitalPCR #NextGenerationSequencing #singlecell #diagnostics

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