[Biomacromolecules] Glycan functionalized microgels for scavenging and specific binding of lectins
Lectins are proteins with a well-defined carbohydrate recognition domain. Multiple microbial proteins such as bacterial toxins possess lectin or lectin-like binding domains to interact with cell membranes that are decorated with glycans recognition motifs. Here, we report a straightforward way to prepare monodisperse and biocompatible polyethylene glycol microgels, which carry glycan motifs for specific binding to lectins. The sugar-functionalized colloids exhibit a wide mesh size and a highly accessible volume. The microgels are prepared via drop-based microfluidics combined with radical polymerization. GSII and ECL are used as model lectins that bind specifically to the corresponding carbohydrates namely GlcNAc and LacNAc. LacNAc microgels bind ECL with a high capacity and high affinity (Kd ~ 0.5 – 1 µM) suggesting multivalent binding of the lectin to the LacNAc-decorated flexible microgel network. Glycan functionalized microgels present a useful tool for lectin scavenging in biomedical applications.
Alexander Jans, Ruben R. Rosencrantz, Ana D. Mandic, Naveed Anwar, Sarah Boesveld, Christian Trautwein, Martin Moeller, Gernot Sellge, Lothar Elling, and Alexander J. C. Kuehne
Biomacromolecules, Just Accepted Manuscript
Publication Date (Web): March 3, 2017
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