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[Protocol] How to make Monodispersed droplets using Flow Focusing Device

Video Description: Here is an example of a Flow Focusing Device. In this video, it is a Co-flow device because there are two water inlets, one for Cell, the other one for lysis buffer. They come into contact first before pinched off by the third channel, which is oil with surfactant so droplet can be stable after generation.

First paper published on how to make a Flow Focusing device is this one:

Formation of dispersions using “flow focusing” in microchannels

Shelley L. Anna, Nathalie Bontoux, and Howard A. Stone

Appl. Phys. Lett. 82, 364 (2003); doi: http://dx.doi.org/10.1063/1.1537519


A flow-focusing geometry is integrated into a microfluidic device and used to study drop formation in liquid–liquid systems. A phase diagram illustrating the drop size as a function of flow rates and flow rate ratios of the two liquids includes one regime where drop size is comparable to orifice width and a second regime where drop size is dictated by the diameter of a thin “focused” thread, so drops much smaller than the orifice are formed. Both monodisperse and polydisperse emulsions can be produced.

Link to the PDF of this paper: http://repository.cmu.edu/cgi/viewcontent.cgi?article=1043&context=cheme


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