• Microfluidics News Admin

[Journal of Experimental Nanoscience ]Harnessing electrical energy for anti-biofilm therapies: effec


Preferentially altering bacterial migration could be a successful approach for augmenting the natural wound-healing process. Inducing electrotactic behaviours through the application of physiologically safe currents is one possible strategy for altering bacterial movement. By controlling bacterial movement at the site of infection, healing times and the severity/extent of bacterial infection could be reduced. Here, we deployed microfluidics and atomic force microscopy to determine the effect of an applied electrical current on bacterial motility and cell morphology in wound pathogens namely Pseudomonas aeruginosa, Escherichia coli, and methicillin-resistant Staphylococcus aureus (MRSA). Nanoscale imaging combined with microfluidic platforms allows for the study of single-cell swimming dynamics. Current values of 0, 0.07, and 0.125 mA were applied to bacteria in suspension. E. coli exhibited an increase in directionality and a drop in mean cellular velocity across all voltages. P. aeruginosa showed a significant decrease in mean cellular velocity at 0.07 mA, while all currents increased the directionality of movement. Electrical current had no statistically significant effect on the width or length of individual bacterial cells. A better understanding of how electrical stimulation affects pathogenic bacteria at wound sites may lead to advancements in electrotherapy and help to identify new alternatives to traditional antimicrobial approaches.

Ryan Berthelot & Suresh Neethirajan

Pages 1-11 | Received 05 Sep 2016, Accepted 15 Feb 2017, Published online: 28 Feb 2017

Journal of Experimental Nanoscience

Link: http://www.tandfonline.com/doi/citedby/10.1080/17458080.2017.1296977?scroll=top&needAccess=true

#bioflim #bacteria #Others #Biologicalapplication

4 views0 comments

Recent Posts

See All

© 2017 by "Microfluidics News".