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[Conference]Pulsed laser activated cell sorter with dielectrophoretic single stream sheathless focus


Abstract:

We report a novel sheathless microfluidic fluorescence-activated cell sorter utilizing size-independent three-dimensional dielectrophoretic (DEP) single stream focusing and pulsed laser activated cell sorting (PLACS). This is realized by fabricating a 3D microfluidic device using two glass substrates with patterned electrodes sandwiching a thin and open PDMS channel. DEP forces are provided along a 4cm-long channel that focuses particles into a single stream passing through a fluorescence detection zone regardless of their sizes and types at high flow speeds. Pulsed laser-induced cavitation bubbles are used to provide rapid (∼30 μs) and precise fluid perturbation to sort out particles upon the detection of fluorescence. High purity sorting has been accomplished at a throughput of 1500 particles sec−1.

Xiongfeng Zhu

Department of Mechanical and Aerospace Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA

Yu-Chun Kung

Department of Mechanical and Aerospace Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA

Ting-Hsiang Wu

NantWorks, LLC, Culver City, CA 90232, USA

Michael A. Teitell

Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA

Pei-Yu Chiou

Department of Mechanical and Aerospace Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA

Conference: Micro Electro Mechanical Systems (MEMS), 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems (MEMS)

Date of Conference: 22-26 Jan. 2017

Date Added to IEEE Xplore: 28 February 2017

DOI: 10.1109/MEMSYS.2017.7863646

Link: http://ieeexplore.ieee.org/abstract/document/7863646/

#digitalmicrofluidics #dielectrophoresis #cellsorting #MEMS #Biologicalapplication

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