Abstract:

This paper describes a dynamic microarray device that facilitates a particle-based multiplex analysis by co-locating multiple, hetero particles at a designated reaction chamber array. The device allows parallel monitoring of multiple reactions among clustered particles. A high-density particle cluster array was formed by utilizing flow-induced deformability of a polydimethylsiloxane (PDMS) membrane that is caused by applied hydrodynamic pressure. By incorporating immiscible oil isolation, particles clusters were isolated from the outer environment, which enabled a cross-contamination-free analysis. We monitored the interactions among clustered hetero particles, without any cross-contamination, by co-locating polystyrene beads and chemical-laden hydrogels within arrayed reaction chambers and isolating them using immiscible oil.

Published in: Micro Electro Mechanical Systems (MEMS), 2017 IEEE 30th International Conference on

Date of Conference: 22-26 Jan. 2017

Date Added to IEEE Xplore: 28 February 2017

DOI: 10.1109/MEMSYS.2017.7863651

Sanghyun Lee

Pohang University of Science and Technology (POSTECH), Republic of Korea

Hojin Kim

Pohang University of Science and Technology (POSTECH), Republic of Korea

Wonhyung Lee

Pohang University of Science and Technology (POSTECH), Republic of Korea

Joonwon Kim

Pohang University of Science and Technology (POSTECH), Republic of Korea

Link: http://ieeexplore.ieee.org/abstract/document/7863651/

#microarray #Others #particle