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[Conference]Lipid bilayer at vertically aligned nanoliter droplets generated by two-layered microflu


Abstract:

We propose a microfluidic device for observable lipid bilayer formation between a pair of vertically aligned aqueous droplets. The device feature includes visibility of the bilayer and variable volume of the droplets, together with sub-microliter volumes of droplets and time-course observation. Referring the metering and storage modules that we recently reported, a pair of droplets was generated in symmetrical two-layered (top and bottom) channels; the droplets then moved into the storage well and contacted with each other from the top and the bottom layers. We demonstrated a bilayer formation using the device and observed crystallization of potassium ferricyanide, which was probably attributed to water permeation through the bilayer.

Published in: Micro Electro Mechanical Systems (MEMS), 2017 IEEE 30th International Conference on

Date of Conference: 22-26 Jan. 2017

Date Added to IEEE Xplore: 28 February 2017

DOI: 10.1109/MEMSYS.2017.7863650

T. Osaki

Kanagawa Academy of Science and Technology, Japan

T. Kaminski

Institute of Physical Chemistry, Polish Academy of Sciences, Poland

K. Kamiya

Kanagawa Academy of Science and Technology, Japan

S. Fujii

Kanagawa Academy of Science and Technology, Japan

N. Misawa

Kanagawa Academy of Science and Technology, Japan

P. Garstecki

Institute of Physical Chemistry, Polish Academy of Sciences, Poland

S. Takeuchi

Kanagawa Academy of Science and Technology, Japan

Link: http://ieeexplore.ieee.org/abstract/document/7863650/

#dropbasedmicrofluidics #MEMS #lipid #microchannels

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