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[Conference]Gene transfer by circulating plasma bubble flow
Abstract:
We have succeeded in injection of plasmid to adherent cells which are suspended in the plasma-bubbles laden circulation flow in a chamber. High-speed plasma-bubbles are generated by glass electrode and the air-liquid interface has a stiction force which draws the gene (plasmid) and stick to the air-liquid interface. The circulating flow increased the chance for cells to contact air-liquid interface of bubbles which enclosed plasma or reactive gas. Finally, the high reactive interface enables gene transfer to cells efficiently. This technology of two-dimensional microfluidic chip contributes an option to the high-throughput gene transfer.
Published in: Micro Electro Mechanical Systems (MEMS), 2017 IEEE 30th International Conference on
Date of Conference: 22-26 Jan. 2017
Date Added to IEEE Xplore: 28 February 2017
DOI: 10.1109/MEMSYS.2017.7863438
Yoko Yamanishi
Kyushu University, Fukuoka, Japan
Ryotaro Tanaka
Kyushu University, Fukuoka, Japan
Yuta Arakawa
Shibaura Institute of Technology, Tokyo, Japan
Yoshimichi Nakatsu
Kyushu University, Fukuoka, Japan
Link: http://ieeexplore.ieee.org/abstract/document/7863438/
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