Abstract:

We have succeeded in injection of plasmid to adherent cells which are suspended in the plasma-bubbles laden circulation flow in a chamber. High-speed plasma-bubbles are generated by glass electrode and the air-liquid interface has a stiction force which draws the gene (plasmid) and stick to the air-liquid interface. The circulating flow increased the chance for cells to contact air-liquid interface of bubbles which enclosed plasma or reactive gas. Finally, the high reactive interface enables gene transfer to cells efficiently. This technology of two-dimensional microfluidic chip contributes an option to the high-throughput gene transfer.

Published in: Micro Electro Mechanical Systems (MEMS), 2017 IEEE 30th International Conference on

Date of Conference: 22-26 Jan. 2017

Date Added to IEEE Xplore: 28 February 2017

DOI: 10.1109/MEMSYS.2017.7863438

Yoko Yamanishi

Kyushu University, Fukuoka, Japan

Ryotaro Tanaka

Kyushu University, Fukuoka, Japan

Yuta Arakawa

Shibaura Institute of Technology, Tokyo, Japan

Yoshimichi Nakatsu

Kyushu University, Fukuoka, Japan

Link: http://ieeexplore.ieee.org/abstract/document/7863438/

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