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[Conference]Direct PCR amplification and in situ imaging based on alginate droplets


Abstract:

A droplet based microfluidic system encapsulating PCR reagent into alginate beads as a new digital PCR solution has been developed, which avoided droplet coalescence and enabled efficient DNA amplification and high-throughput fluorescent imaging. We also compared two trapping methods using hydrodynamic traps and micro-sieves, and indicated the advantages of latter for higher trapping rate and no droplet clogging. These results provide new insights and tools for on-chip digital PCR and are expected to attract broad interest for applications in droplet microfluidics.

Published in: Micro Electro Mechanical Systems (MEMS), 2017 IEEE 30th International Conference on

Date of Conference: 22-26 Jan. 2017

Date Added to IEEE Xplore: 28 February 2017

DOI: 10.1109/MEMSYS.2017.7863644

Lijun Li

Shenzhen institute of Advanced Technology, Chinese Academy of Science, Shenzhen, China

Linfen Yu

Shenzhen institute of Advanced Technology, Chinese Academy of Science, Shenzhen, China

Tianzhun Wu

Shenzhen institute of Advanced Technology, Chinese Academy of Science, Shenzhen, China

Link: http://ieeexplore.ieee.org/abstract/document/7863644/

#DigitalPCR #alginate #dropbasedmicrofluidics #insituimaging

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