Abstract:

We present binding properties of biomolecules and their dependence on ionic strength and temperature using a microfluidic aptameric graphene field-effect transistor (FET) nanosensor. Aptamer-immobilized graphene is used to recognize target molecules, and the resulting changes in graphene conductance is measured to study the binding kinetics of biomolecules. Binding properties of aptamer-protein interactions at different ionic strength and temperature are investigated, yielding insight into the pharmacologic basis of biomolecular recognition.

Published in: Micro Electro Mechanical Systems (MEMS), 2017 IEEE 30th International Conference on MEMS

Date of Conference: 22-26 Jan. 2017

Date Added to IEEE Xplore: 28 February 2017

DOI: 10.1109/MEMSYS.2017.7863469

Xuejun Wang

Department of Mechanical Engineering, Columbia University, New York, NY 10027, USA

Zhuang Hao

Department of Mechanical Engineering, Columbia University, New York, NY 10027, USA

Wenjun Zhuang

Department of Mechanical Engineering, Columbia University, New York, NY 10027, USA

Qiao Lin

Department of Mechanical Engineering, Columbia University, New York, NY 10027, USA

Link: http://ieeexplore.ieee.org/abstract/document/7863469/

#sensor #graphene #MEMS