• Microfluidics News Admin

[Conference]: A microfluidic device for antimicrobial susceptibility testing of combined antibiotics


Abstract:

A simple microfluidic device which can perform antimicrobial susceptibility testing (AST) of combined antibiotics against antibiotics-resistant bacteria has been presented. A new approach to determine the fractional inhibitory concentration (FIC) index of two antibiotics by performing broth dilutions automatically was proposed and its performance was highly consistent with manual operation. The experimental results showed that the developed device could achieve on-chip dispensation/dilution/mixing accurately and interpret AST results visually. A test using two common antibiotics (vancomycin and ceftazidime) was performed on one clinical isolate of vancomycin-intermediate Staphylococcus aureus (VISA) based on the FIC indices. It is the first time that a microfluidic device was demonstrated to be capable of determining the FIC index of combined antibiotics against bacteria by performing AST automatically. With its high flexibility and reliability, the proposed device provides a simple method to explore drug cocktails for combined antibiotic therapy.

Published in: Micro Electro Mechanical Systems (MEMS), 2017 IEEE 30th International Conference on

Date of Conference: 22-26 Jan. 2017

Date Added to IEEE Xplore: 28 February 2017

DOI: 10.1109/MEMSYS.2017.7863480

Wen-Bin Lee

Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan

Wen-Hsin Chang

Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan

Huey-Ling You

Laboratory Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University, Kaohsiung, Taiwan

Mel S. Lee

Department of Orthopaedic Surgery, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University, Kaohsiung, Taiwan

Gwo-Bin Lee

Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan

Link: http://ieeexplore.ieee.org/abstract/document/7863480/

#susceptibility #bacteria #antibiotics #Biologicalapplication #MEMS

Recent Posts

See All

© 2017 by "Microfluidics News".