Abstract:

We present a novel μrespirometer to determine the oxygen consumption rate (OCR) of mammalian cells. The oxygen concentration is measured with the fluorescent dye PtTFPP in a polystyrene-matrix. This film is integrated into a closed microfluidic chip made out of only oxygen-impermeable materials like glass and silicon. This results in a low drift and thus allows long-term measurements with living mammalian cells. With this chip, we determined the OCR of basophil RS-ATL8 cells as 32 amol/(celhs).

Published in: Micro Electro Mechanical Systems (MEMS), 2017 IEEE 30th International Conference on MEMS

Date of Conference: 22-26 Jan. 2017

Date Added to IEEE Xplore: 28 February 2017

DOI: 10.1109/MEMSYS.2017.7863430

Frank Bunge

Institute for Microsensors, -actuators and -systems (IMSAS), University of Bremen, Germany

Sander van den Driesche

Institute for Microsensors, -actuators and -systems (IMSAS), University of Bremen, Germany

Anya Waite

Alfred-Wegener-Institute, Bremerhaven, Germany

Ursula Mirastschijski

Dept. of Plastic, Reconstructive and Aesthetic Surgery, Klinikum Bremen-Mitte and, Centre for Biomolecular Interactions Bremen, University of Bremen, Germany

Michael J. Vellekoop

Institute for Microsensors, -actuators and -systems (IMSAS), University of Bremen, Germany

Link: http://ieeexplore.ieee.org/abstract/document/7863430/

#cell #respirometer #MEMS #Biologicalapplication