Microfluidics News AdminAug 22, 20172 min[Physical Chemistry Chemical Physics] Gradient-free determination of isoelectric points of proteins Abstract: The isoelectric point (pI) of a protein is a key characteristic that influences its overall electrostatic behaviour. The majority of conventional methods for the determination of the isoelectric point of a molecule rely on the use of spatial gradients in pH, although significant practical challenges are associated with such techniques, notably the difficulty in generating a stable and well controlled pH gradient. Here, we introduce a gradient-free approach, exploiti
Microfluidics News AdminMar 21, 20171 min[Journal of Membrane Science] A microfluidic system for studying particle deposition during ultrafilAbstract: A significant limitation in the application of membrane filtration is fouling caused by particle deposition. To better understand the key mechanisms of particle deposition, a microfluidic filtration system was developed that combined confocal scanning laser microscopy (CSLM) and a dual syringe pump flow control setup. It has been used successfully to visualize in real-time and quantify the deposition of latex particles (0.4 µm) onto membranes during cross-flow ultra
