Microfluidics News AdminAug 8, 20181 min[Lab on a chip] Charge-controlled microfluidic formation of lipid-based single- and multicompartmentAbstract: In this manuscript, we introduce a simple, off-the-shelf approach for the on-demand creation of giant unilamellar vesicles (GUVs) or multicompartment synthetic cell model systems in a high-throughput manner. To achieve this, we use microfluidics to encapsulate small unilamellar vesicles in block-copolymer surfactant-stabilized water-in-oil droplets. By tuning the charge of the inner droplet interface, adsorption of lipids can be either inhibited, leading to multicom
Microfluidics News AdminApr 1, 20171 min[Bio-Optics: Design and Application] Label-free dynamic volumetric imaging of deforming giant unilamAbstract: The flow of giant unilamellar vesicles (GUVs) in capillaries has direct relevance to the understanding of blood rheology in microcirculation. We demonstrate a dynamic volumetric imaging of multiple GUVs undergoing deformation in a microfluidic system Xuefei He, Katharina Gaus, and Woei Ming Lee Optics in the Life Sciences Congress OSA Technical Digest (online) (Optical Society of America, 2017), paper BoS2A.2 •https://doi.org/10.1364/BODA.2017.BoS2A.2 Link: https://
Microfluidics News AdminMar 17, 20171 min[Biosensors and Biodetection] Functionalized Vesicles by Microfluidic DeviceAbstract: In recent years, lipid vesicles have become popular vehicles for the creation of biosensors. Vesicles can hold reaction components within a selective permeable membrane that provides an ideal environment for membrane protein biosensing elements. The lipid bilayer allows a protein to retain its native structure and function, and the membrane fluidity can allow for conformational changes and physiological interactions with target analytes. Here, we present two methods
